Fig. 8: Profiling stressor combinations with Kinbiont.

Graphical representation of the first two levels of the maximum depth decision trees for the different kinetic parameters inferred for the mixture of strains A—C and for the strain N. soli D—E. Dashed branches mark leaves where the chemicals from the parent node are absent; solid branches mark leaves where they are present. For every leaf, violin plots (with individual points and an internal box-plot showing the 0.25, 0.50, and 0.75 quantiles) display the distribution of the corresponding growth parameter. Two-sided Wilcoxon P values accompanying each pair of violins quantify how significantly the split separates the group means. In addition to identifying the chemicals with the most significant statistical effects - like the pronounced impact of Oxytetracycline on the maximum biomass concentration of the mixture in panel B—this graphical representation can also be used to explore chemical interactions. For instance, it can detect synergistic interactions between Oxytetracycline and Imidacloprid in E, and antagonistic interactions between Tebuconazole and Oxytetracycline in D and F.