Fig. 1: Map-based cloning of AcEGY3 mediating thermotolerance of kiwifruit. | Nature Communications

Fig. 1: Map-based cloning of AcEGY3 mediating thermotolerance of kiwifruit.

From: Natural variation of AcEGY3 mediates chloroplastic ROS homeostasis to confer kiwifruit thermotolerance

Fig. 1

Shoot phenotypes (a), leaf survival rates (b), and net photosynthetic rates (c) of A. eriantha “MH-Z11” and an offspring line of A. chinesis cv. “Hongyang” (HY0809) after treatment for 8 h in a 40 °C growth chamber and after recovery for 2 days (2 d) in a 23 °C growth chamber. d BSA sequencing and QTL mapping of a backcross population between HY and MH-Z11 by using the GradePool-seq method. The mapping region (from 14820000 to 15200000) with the highest ratio value is located on chromosome group 2. The Actinidia02441 (AcEGY3) gene was highlighted in red. Heatmap derived from transcriptomic analysis of the annotated genes in the identified region (e) and qRT-PCR verification of AcEGY3 (f) in MH-Z11 and HY plants treated with 40 °C for 0, 1, 3, and 8 h. The Actinidia02441 (AcEGY3) gene was highlighted in red. In (b, c, f), asterisks“***” indicate significant differences at p < 0.001 by two-tailed Student’s t-test. Data represent mean ± SD (n = 6 biological replicates). Source data are provided as a Source Data file.

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