Fig. 4: Attribution of soil Rank I ARGs and the connectivity of soil with other habitats.

a The attribution of soil Rank I ARGs. Total 8388 E. coli isolates were included in the FEAST analysis. 999 rounds of random sampling were carried out, and 38 genomes per habitat per period were included in each calculation. In the boxplots of panels, hinges indicate the 25th, 50th, and 75th percentiles, whiskers indicate 1.5× interquartile ranges, dots indicate the average value from each random sampling. Significant comparisons (two-sided t test) between different temperature and different groups were calculated and detailed p value and T stat were provided in the Supplementary Data 11. b The impact of different habitats on the composition of soil Rank I ARGs. In the boxplots of panels, hinges indicate the 25th, 50th, and 75th percentiles, whiskers indicate 1.5× interquartile ranges, and dots indicate the average value from each random sampling. Nine hundred and ninety-nine rounds of random sampling were carried out, and 38 genomes per habitat per period were included in each calculation. Significant comparisons (two-sided t test) between different temperature and different groups were calculated and detailed p value and T stat were provided in the Supplementary Data 11. The correlation was statistically tested using linear model (two-sided). R² and slope is calculated from the linear model. Cattle feces: p < 0.017, human: p < 0.026, chicken feces: p < 0.034. c Workflow for calculating connectivity. d The connectivity of soil with other habitats over time. 999 rounds of random sampling were carried out, and 30 genomes per habitat per period were included in each calculation. The results used eptA (one gene type of Rank I ARGs) as an example. In the boxplots of panels, hinges indicate the 25th, 50th, and 75th percentiles, whiskers indicate 1.5× interquartile ranges, dots indicate the average value from each random sampling. Significant comparisons (two-sided t test) between different temperature and different groups were calculated and detailed p value and T stat were provided in the Supplementary Data 12. The correlation was statistically tested using linear model (two-sided). R² and slope is calculated from the linear model. Human: p < 0.0034, chicken feces: p < 0.0065, pig feces: p < 0.031.