Fig. 4: Linc-7 is localized to perinuclear foci with FBF-2 and PGL-1. | Nature Communications

Fig. 4: Linc-7 is localized to perinuclear foci with FBF-2 and PGL-1.

From: Three lncRNAs promote PUM protein condensation and germline differentiation

Fig. 4

a, b Late progenitor zone (a) and LZ (b) nuclei stained with linc-7 smFISH (red), GFP::FBF-2 (green) and DAPI (blue). c Average percentage of linc-7 perinuclear foci that co-localize with GFP::FBF-2. d Average percentage of GFP::FBF-2 perinuclear granules that co-localize with linc-7. e, f Late progenitor zone (e) and LZ (f) nuclei stained with linc-7 smFISH (red), PGL-1::GFP (green) and DAPI (blue). g Average percentage of linc-7 perinuclear foci that colocalize with PGL-1::GFP. h Average percentage of PGL-1::GFP perinuclear condensates that colocalize with linc-7. N values represent cells and are marked on the columns. Scale bar = 5 μM. Arrow: colocalized foci. Arrowhead: protein focus that do not co-localize with linc-7. Two tailed Mann Whitney P value: *<0.05, ****<0.0001. lPZ: late progenitor zone. LZ: Leptotene/Zygotene. Error bars indicate standard error. Gray x’s on the plot represent individual measurements. Source data are provided as a Source Data file.

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