Fig. 3: Impact of caspofungin on C. albicans and C. auris.
a Growth profile of C. albicans SC5314 (black), C. auris AR386 (orange), and C. auris I.3 (purple) in response to varying concentrations of caspofungin. Percentages represent the comparison of OD600 values between cultures grown after 24 h with and without caspofungin. b Cell wall thickness of C. albicans SC5314 and two C. auris strains. For each strain the untreated (apo; pale orange) and caspofungin-treated (CAS; blue) are compared. The mean ± s.d are provided. n = 100 for each sample. Black rectangles denote the interquartile range (IQR) from 25th to 75th percentile. White circles represent the mean of the dataset, and black vertical lines correspond to 1.5IQR. Statistical analysis was performed using a t-test with one tail comparison between apo and drug-treated samples for each strain. Statistically significant: *p-value ≤ 0.05. c 1D 13C cross polarization (CP) spectra of apo (top; orange) and caspofungin-treated (bottom; blue) samples of three Candida strains. d 2D 13C-13C 53 ms CORD spectra of C. albicans SC5314 (top), C. auris AR386 (middle), and C. auris I.3 (bottom), with and without exposure to caspofungin. Characteristic signals of chitin (Ch), β−1,6-glucan (H), and mannan (Mn) are highlighted within dashed line boxes in orange, black, and light brown, respectively. e Molar composition of carbohydrate components in the rigid and mobile fractions within Candida cell walls. The percentage values were estimated from the volumes of resolved cross-peaks in 2D CORD spectra for the rigid fraction and the volumes of resolved signals in 2D DP J-INADEQUATE spectra for the mobile molecules. Source data are provided as a Source Data file.
