Fig. 6: Susceptibility to micafungin and caspofungin in KRE6 mutant strains of C. auris.
Relative expression of genes involved in cell wall biosynthesis (KRE6a, KRE6b, FKS1 and CHS1) by real-time reverse transcription RT-PCR are compared for (a) KRE6a and KRE6b deletion and complemented strains, as well as (b) KRE6a and KRE6b overexpressing strains. Black: AR386; cyan: kre6aΔ; orange: kre6bΔ; red: kre6aΔ::KRE6a; dark blue: kre6bΔ::KRE6b; yellow: PADH1_KRE6a; purple: PADH1_KRE6b. Results are expressed as fold-change compared to the wild-type AR386. Bars represent the mean with s.d. of three biological replicates. Data points are represented by open circles. Statistical analysis was performed using a t-test with two tails compared to AR386. Statistically significant: *p-value ≤ 0.05. **p-value ≤ 0.01. c Susceptibility to micafungin (left) and caspofungin (right) was performed by spotting assays in all the KRE6a/b mutants. Spotting assays were performed with serial dilutions of yeast cells spotted on YEPD agar plates containing different concentration of micafungin or caspofungin. Plates were incubated for 24 h at 37 °C. d Susceptibility to micafungin and caspofungin performed using microbroth dilution assay (EUCAST protocol) in all the KRE6a/b mutants. Growth is expressed as the percentage of OD600 compared to that of the wild-type AR386 in the absence of drug. Open circle represent the mean of biological duplicates and error bars are s.d. Source data are provided as a Source Data file.
