Fig. 7: Modulated cell wall composition in KRE6 mutant strains of C. auris. | Nature Communications

Fig. 7: Modulated cell wall composition in KRE6 mutant strains of C. auris.

From: Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR

Fig. 7

a 1D 13C CP spectra of the four mutant samples prepared without (blue) and with (magenta) 0.5 µg/mL caspofungin. b 2D 13C CORD spectra of kre6aΔ (left) and kre6bΔ (right) samples prepared without (blue) and with (magenta) 0.5 µg/mL caspofungin. Signature spectral regions are highlighted by dashed line boxes for chitin (Ch; orange), β-1,3-glucan (B; blue), and mannan (Mn; brown). c Molar composition of rigid (left) and mobile (right) carbohydrates estimated from peak volumes in 2D CORD and DP J-INADEQUATE spectra, respectively. Black: AR386; yellow: PADH1_KRE6a; purple: PADH1_KRE6b; blue: kre6aΔ; orange: kre6bΔ. The corresponding pale color is used for caspofungin-treated sample of each strain. For each carbohydrate, data is shown for tens samples: the AR386 strain and four mutants prepared with and without caspofungin. Asterisks highlight the decreased content of β−1,6-glucan in KRE6 deletion mutants, which are recovered by caspofungin treatment. Source data are provided as a Source Data file.

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