Fig. 1: Development of CKAR3 in cultured cells.
From: A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo
a Schematic of previous FRET-based PKC sensors and CKAR3. FHA1 and FHA2: Forkhead-associated domains 1 and 2, respectively; PH: Pleckstrin homology domain; DEP: disheveled, Egl-10, pleckstrin; NES: nucleus exportation sequence. b Representative Δlifetime images of the tested sensors at rest and after stimulation with 1 μM PDBu and 1 μM ionomycin (iono.). c Averaged response (ΔLT) traces of existing sensors. Quantification of existing sensors (d; at the gray window of panel c) and candidate variants (e). From left to right, n (cells/coverslip) = 11/4, 9/4, 6/3, 10/4, and 15/6 in panel (d), and 9/4, 7/3, 7/3, 10/3, 6/3, and 14/4 in panel e. f, Schematic of CKAR3 sensor. Note that only a partial substrate sequence was included in the schematic due to space constraints. The schematic was modified from (a) in ref. 31 with permission. Where applicable throughout the figure, mean (dark lines and bars) and s.e.m. (shaded area and error bars) are shown. Source data are provided as a Source Data file.
