Fig. 4: In vivo CKAR3 imaging revealed cell-specific, tonic PKC activity.
From: A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo
Representative intensity and lifetime images (a) and quantifications of basal lifetimes (b) of CKAR3 or mutCKAR3 in L2/3 neurons of the M1 and V1 cortices and Purkinje cells of cerebellar lobule VI in awake mice, as indicated. LT: lifetime; int.: intensity. In panel (b) from left to right, n (cells/mice) = 132/7, 240/5, 82/3, and 110/3. For comparison between mut. (M1) and M1, two-sided Mann–Whitney U test was used [U = 13087, p = 0.006]; for comparison among M1, V1 and lobule VI, Kruskal-Wallis test [H(2) = 173.6, p = 3.2 × 10–38] followed by corrected Dunn’s post hoc test [M1 vs. V1: p = 2.4 × 10–9, M1 vs. lobule VI: p = 1.1 × 10–37, V1 vs. lobule VI: p = 4.2 × 10–6] was used. c Correlation of basal lifetimes with linear fit (solid line) and 95% confidence intervals (dashed lines) of the same M1 neurons across approximately a week. n (cells/mice) = 65/4, p = 3.2 × 10–13, F-test. d Basal lifetime change of CKAR3 and mutCKAR3 induced by indicated drugs. From left to right, n (cells) = 60, 85, 60, 84, 60, and 70 from 4 mice. Two-sided Mann–Whitney U test was used [veh.: U = 2535, p = 0.95; scop.: U = 848, p = 4.4 × 10–10; JNJ: U = 2420, p = 0.69]. Throughout the figure, horizontal black lines indicate mean and s.e.m.; **: p < 0.01, ***: p < 0.001. Source data are provided as a Source Data file.
