Fig. 1: Overview of sgRNA and split sgRNA strategies for Cas12b.
From: Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b
a The complete sequence and potential secondary structure of sgRNA for Cas12b. b Illustration of the full-length sgRNA designed for Cas12b functionality. c Overview of the traditional split strategy, incorporating tracrRNA and crRNA for Cas12b, where the blue segment denotes tracrRNA and the pink segment represents crRNA. Our currently developed split sgRNA strategies for Cas12b are illustrated as follows: d tracrDR+Spacer and e tracrRNA+DR+Spacer. The red region indicates the universal DR region (16 nt), while the green region depicts the interchangeable Spacer region (18–23 nt).
