Fig. 3: The tracrDR+Spacer and tracrRNA+DR+Spacer strategies are employed to detect distinct nucleic acid targets through simple modifications to the Spacer region.
From: Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b
The tracrDR+Spacer strategy was tested with EBV-specific Spacers against various concentrations of EBV targets, yielding fluorescence detection values (a) and their corresponding linear relationship plot (b). Data are shown as mean value +/− SD (n = 3, biologically independent samples). Similarly, the tracrRNA+DR+Spacer strategy was assessed using the EBV Spacer with varying concentrations of EBV targets, as indicated by the fluorescence detection values (c) and the corresponding linear relationship plot (d). Data are shown as mean value +/− SD (n = 3, biologically independent samples). e A summary table detailing the linear ranges and detection limits for different targets (EBV, HCV, MPXV) as determined by the tracrDR+Spacer and tracrRNA+DR+Spacer strategies, accomplished by simply replacing the relevant Spacer regions. Source data are provided as a Source Data file.
