Fig. 2: Multigenerational adaptation of animals previously adapted to OP, BS, SM and PB bacteria.
From: C. elegans sperm and oocytes differentially transmit diet-induced adaptations to their progeny
a–d Quartile box plots (whisker 1.5*IQR) of brood size of animals previously adapted to (a), OP (light blue background), b BS (light purple), c SM (light orange), and (d) PB (light green), and then transferred to each of the indicated new bacterial foods. Three biological replicates, n = 10 complete broods per each food for each replicate (except n = 9 for SM- > BS, rep 3 G9, G10 and SM- > SM rep 3, G10). e, h, k The percentage of 50 embryos from G0 (n = 5) and G10 (n = 5) hermaphrodites grown on each food that hatched. One-tailed t-test compared to “same-to-same” grown worms. f, i, l, The percentage of 10 self-sperm-depleted G0 (n = 3) or G10 (n = 2) hermaphrodites grown on the indicated food that produced cross-progeny when mated with OP grown wild-type males. In two G0 (BS- > BS, PB- > OP) and two G10 (BS- > OP BS- > OP) experiments only 9 hermaphrodites were assayed. P values were calculated by one-tailed t-test compared to “same-to-same” grown worms. g, j, m, The percent of self-progeny males from the sum of all progeny (inset numbers) produced by five G0 or G10 animals grown on the indicated food. P values were calculated by Fisher exact test, compared to “same-to-same” grown worms. Approximate statistical significance is indicated on some panels. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. Source data and numerical P values are provided as a Source Data file.
