Table 1 Products distribution of quinoline catalyzed by mutants of P450BM3

From: Regulating the N-oxidation selectivity of P450BM3 monooxygenases for N-heterocycles through computer-assisted structure-guided design

Mutants

Conv.a

(%)

N-oxidation

sele.(%)

5-OH

sele.(%)

3-OH

sele.(%)

Othersb

(%)

F87G

39.4

82.7

4.8

1.1

11.4

F87V

31.3

73.8

5.6

2.5

18.1

F87A

15.6

42.6

16.5

n.d.

40.9

F87G/A264G

64.4

92.7

3.7

n.d.

7.3

F87G/A264G/A328L

68.2

98.5

n.d.

n.d.

1.5

  1. 5-OH 5-hydroquinoline, 3-OH 3-hydroquinoline. ‘sele.’ represents regioselectivity, and ‘conv.’ represents conversion of substrate. Reactions were conducted in three independent experiments (n = 3).
  2. aReaction conditions: 1 mM quinoline in 1 mL K2HPO4/KH2PO4 (50 mM) buffer pH 8.0 for 16 h, 220 rpm, 30 °C.
  3. bUnknown compounds.
Back to article page