Fig. 2: Optimization of chemical circularization of two RNA oligonucleotides (35 nt).
From: Chemical circularization of in vitro transcribed RNA for exploring circular mRNA design
A PAGE analysis of the circularization reaction of (EDA)RNA01 and (EDA)RNA02. RNAs were circularized at concentrations of 0.3, 1.2, or 12 µM, at 4 or 20 °C, with (+ΔT) or without (−ΔT) thermal refolding (rapid heating and subsequent slow cooling) prior to circularization. S refers to an untreated RNA substrate. Data presented is representative of one independent experiment. B–D Minimum-free-energy (MFE) secondary structure (2D) models (predicted with RNAfold web server) and tertiary structure (3D) of predicted models (RNA Composer web server) for RNA01 (B), RNA02 (C), and RNA02 dimer (D). Content of mRNA species was quantified using CLIQS (Core Laboratory Image Quantification Software).
