Fig. 4: Electrophoretic and enzymatic methods for the analysis of chem-circRNA.
From: Chemical circularization of in vitro transcribed RNA for exploring circular mRNA design
A–C Resolution of circular RNAs and their linear precursors based on molecular size (A RNA01 35 nt, B RNA03 276 nt, C RNA04 760 nt), gel density, and cross-linking degree of denaturing polyacrylamide. D, E Treatment of linear and circular mRNA with endo- and exonucleases (D RNase H—RH and E RNase R—RR, respectively) to confirm the circular topology of the reaction products. A–E Data presented is representative of one independent experiment.
