Fig. 7: Chem-circRNAs with endocyclic cap structure have superior translational properties.
From: Chemical circularization of in vitro transcribed RNA for exploring circular mRNA design
A–H Translation activity and protein expression profiles of enzymatically circularized and chem-circRNAs derived from four RNA templates (RNA₁₂–RNA₁₅) in A549, HEK 293 T (HEK), HeLa, and HepG2 (HEP) cells. Circular RNAs were generated from linear precursors containing either a 5′ monophosphate (p-) or an EP₅Cap structure and encoded GLuc. A, C, E, G Show total protein output from transfected cells after 168 h, normalized to expression from the canonical linear Cap1-RNA₁₁ (=1); B, D, F, H Show time-course of GLuc expression in A549 cells transfected with indicated constructs. A, C, E, G Data represent mean ± SD of three biological replicates (n = 3), P values were calculated using unpaired Student’s t test. Source data are provided as a Source Data file.
