Fig. 4: LIN-39 promotes longevity under reduced IIS in a manner dependent on cholinergic VC neurons and the TF DAF-16/FOXO. | Nature Communications

Fig. 4: LIN-39 promotes longevity under reduced IIS in a manner dependent on cholinergic VC neurons and the TF DAF-16/FOXO.

From: LIN-39 is a neuron-specific developmental determinant of longevity in Caenorhabditis elegans with reduced insulin signaling

Fig. 4

a, b distNC-localized DARs opened by LIN-39 in daf-2(e1370) mutants were assigned to their closest neighboring gene, resulting in a list of genes predicted to be regulated by LIN-39. a A barplot showing the enrichment of these LIN-39-regulated genes in genes differentially regulated with age in various tissues, as reported in ref. 37. b A barplot showing the enrichment of these LIN-39-regulated genes in genes expressed in various C. elegans neuron classes, as reported in refs. 38,39. c, d Kaplan–Meier survival estimates with 95% confidence intervals for daf-2 mutants deficient in GABA signaling (daf-2(e1370); unc-25(e156) animals) (c) or ACh signaling (daf-2(e1370); unc-17(e245) animals) (d) grown from the L1 stage on the indicated RNAi bacteria. (n = 106 (daf-2; control RNAi), 92 (daf-2; lin-39 RNAi), 156 (daf-2; unc-25; control RNAi), 143 (daf-2; unc-25; lin-39 RNAi), 163 (daf-2; unc-17; control RNAi), 195 (daf-2; unc-17; lin-39 RNAi); for results from a second independent biological replicate experiment, see Supplementary Table S20). e Kaplan–Meier survival estimates with 95% confidence intervals for daf-2(e1370); eri-1(mg366) and daf-16(mgDf47); daf-2(e1370); eri-1(mg366) mutants grown from the L1 stage on the indicated RNAi bacteria. (n = 133 (daf-2; control RNAi), 291 (daf-2; lin-39 RNAi), 171 (daf-2; daf-16; control RNAi), 193 (daf-2; daf-16; lin-39 RNAi); for results from a second independent biological replicate experiment, see Supplementary Table S21). f, g Kaplan–Meier survival estimates with 95% confidence intervals for eri-1(mg366) and daf-2(e1370); eri-1(mg366) mutant male animals grown from the L1 stage on the indicated RNAi bacteria. (n = 20 (eri-1; control RNAi), 10 (eri-1; daf-16 RNAi), 17 (eri-1; lin-39 RNAi), 66 (daf-2; eri-1; control RNAi), 55 (daf-2; eri-1; daf-16 RNAi), 69 (daf-2; eri-1; lin-39 RNAi); results are derived from combination of two independent biological replicate experiments with consistent results). h Barplots showing functional enrichment analyses of the genes up- or downregulated in VC neurons under reduced IIS. Some terms were adapted or shortened for improved readability (see Supplementary Table S26 for the original terms). Boxes state changes in median lifespan and the associated p-values (based on two-sided log-rank tests) for the indicated comparisons. FDR False discovery rate, rep: replicate. Source data are provided as a Source Data file.

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