Fig. 4: Two reciprocally connected interneurons are oppositely modulated by the feeding state. | Nature Communications

Fig. 4: Two reciprocally connected interneurons are oppositely modulated by the feeding state.

From: Feeding state-dependent neuropeptidergic modulation of reciprocally interconnected inhibitory neurons biases sensorimotor decisions in Drosophila

Fig. 4

a–d Griddle-2 calcium responses to mechanical stimulations in larvae fed on standard food and sucrose (SS00918/UAS-GCaMP6s). a Imaging schematic. b Responses of different individuals, (c) mean calcium response averaged during the stimulus. d Mean Griddle-2calcium trace of over time +/− SEM. e–h Handle-b calcium responses to mechanical stimulations in larvae fed on standard food or on sucrose (SS00888/UAS-GCaMP6s). e Imaging schematic. f Responses of different individuals. g Mean calcium response averaged during the stimulus. h Mean Handle-b calcium trace over time +/− SEM. i–t Reciprocal inhibition between Handle-b and Griddle-2. i, j model simulation, trajectories of LNa activity upon Hb inactivation in fed (i, modeled as rmax_iLNa = 20 a.u.) and sucrose condition (j, modeled as rmax_iLNa = 18 a.u.) Silencing Hb increases activity in iLNa, transiently for low w_iLNa and at steady-state for high w_iLNa, more so in the sucrose than in the fed model. k Imaging schematic. l, m mean Griddle-2 calcium trace over time +/− SEM, with (SS0888-Gal4>UAS-TNT 55C05-LexA>LexAop-GCaMP6s) or without Handle-b inactivation (+/UAS-TNT 55C05-LexA>LexAop-GCaMP6s), in larvae fed on standard food (l) or on sucrose (m). n mean calcium response averaged during the stimulus. o, p model simulation, trajectories of Hb activity upon iLNa inactivation in fed (o, modeled as i_Hb = 0 a.u.) and sucrose condition (p, modeled as i_Hb = 10 a.u.). Silencing iLNa increases strongly the activity of Hb across w_iLNa values and for both models. q Imaging schematic. r, s mean Handle-b calcium trace over time +/− SEM, with (55C05-LexA > LexAop-TNT 22E09-Gal4>UAS-GCaMP6s) or without Handle-b inactivation (+/LexAop-TNT 22E09-Gal4 > UAS-GCaMP6s), in larvae fed larvae (r) or sucrose-fed larvae (s). t mean calcium response averaged during the stimulus. c, g, n, t White line represents the mean, white dot represents the median, colored dots represent individual data points. d,h,l,m,r,s The green dashed line corresponds to stimulation. (Statistics: c, g, n, t two-tailed T-test; ***p < 0.001, **p < 0.01, *p < 0.05). See also Supplementary Figs. 4 and  5. The source data and p-values are provided in Source Data 5 and 6.

Back to article page