Fig. 7: sNPF signaling inhibits Handle-b and facilitates Griddle-2 mechanosensory responses. | Nature Communications

Fig. 7: sNPF signaling inhibits Handle-b and facilitates Griddle-2 mechanosensory responses.

From: Feeding state-dependent neuropeptidergic modulation of reciprocally interconnected inhibitory neurons biases sensorimotor decisions in Drosophila

Fig. 7

a–d Immunohistochemistry labeling for sNPFR in Handle-b. 60E02-LexA drives LexAop-jRGECO1a in Handle-b (magenta), and UAS-GCaMP6s is expressed under the control of the sNPFR promoter using a T2A-Gal4 construct (green). Antibodies against GFP and dsRed were used to increase detection sensitivity. e–g Calcium responses in Handle-b upon sNPFR knockdown (GMR_SS00888 > UAS-GCaMP6s; UAS-sNPFR-RNAi) compared to a control (GMR_SS00888 > UAS-GCaMP6s) in Handle-b. e calcium responses of Handle-b from different individuals and trials. One line is a trial. f mean calcium response averaged during the stimulus. g mean calcium trace of Handle-b over time +/− SEM. h–k Behavior in response to air-puff upon sNPFR knockdown in Handle-b (SS00888 > sNPFR-RNAi) compared to the control. l–o Griddle-2 immunostaining for sNPFR expression. 55C05-LexA line drives LexAop-jRGECO1a in Griddle-2(magenta), and UAS-GCaMP6s is expressed under the control of the sNPFR promoter using a T2A-Gal4 construct (green). Antibodies against GFP and dsRed were used to increase detection sensitivity. p–r Calcium responses in Griddle-2 upon sNPFR knockdown (SS_TJ001 > UAS-GCaMP6s; UAS-sNPFR-RNAi) compared to the control (SS_TJ001 > UAS-GCaMP6s). p calcium responses of Griddle-2 from different individuals and trials. One line is a trial. q mean calcium response averaged during the stimulus. r mean calcium trace of Griddle-2 over time +/− SEM. s–v Behavior in response to air-puff upon sNPFR knockdown in Griddle-2 (SS_TJ001 > sNPFR-RNAi) compared to the control. f, q White line represents the mean, white dot represents the median, colored dots represent individual data points. g, r The green dashed line corresponds to the stimulus. h–j, s–u Hunch and Static-bend are presented as population probability and 95 % confidence interval, Head Cast as post-stimulus probability corrected by baseline probability. k, v Behavioral transitions over the first ten seconds of stimulation (Maximum likelihood test (one-sided, chi-square approximation)). (Statistics: f, q two-tailed T-test; h, i, s, t Chi-square (one-sided) test; j, u Numerical simulation test; k, v Maximum likelihood test (one-sided, chi-square approximation); ***p < 0.001, **p < 0.01, *p < 0.05). See also Supplementary Figs. 7, 9. The source data and p-values are provided in Source Data 2, 3, 5 and 6.

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