Fig. 7: Blocking of galectin-9 controls tumor growth in the TCL1 mouse model. | Nature Communications

Fig. 7: Blocking of galectin-9 controls tumor growth in the TCL1 mouse model.

From: Integrative multi-omics reveals a regulatory and exhausted T-cell landscape in CLL and identifies galectin-9 as an immunotherapy target

Fig. 7

A UMAP plot of 6,201 cells from the spleens of 2 mice after adoptive transfer of TCL1 leukemia (TCL1 AT) analyzed by scRNA-seq identifying 13 clusters, including 11 T-cell clusters, CLL cells and myeloid cells. B Dot plot of the expression of marker genes in the 13 cell clusters. C Representative contour plot and percentage of galectin-9+ (Gal9+) B cells and CLL cells from spleen of wild-type control (WT; n = 5) and TCL1 AT (n = 5) mice, respectively. D Percentage of TIM3+ cells out of CD8 TN, CD8 TM (memory cells), CD8 TEF (effector cells), CD4 TCONV (conventional), and TREG T cells in spleen of WT (n = 5) and TCL1 AT (n = 5) mice measured by flow cytometry. E Schematic diagram of treatment of TCL1 AT mice with galectin-9-blocking antibody (α-Gal9). Analyses of T, myeloid and CLL cells were performed 7 weeks after treatment start in isotype antibody- (n = 11) and α-Gal9-treated (n = 8) mice. Created in BioRender. Floerchinger, A. (2025) https://BioRender.com/x4or7yz. F Absolute number of CD19+ CD5+ CLL cells in blood of isotype antibody- (n = 11) and α-Gal9-treated (n = 8) mice. G Spleen weight of isotype antibody- (n = 11) and α-Gal9-treated (n = 8) mice. H Representative contour plot and percentage of CD39+ cells out of CD8+ T cells from spleen of isotype antibody- (n = 11) and α-Gal9-treated (n = 8) mice. I Representative contour plot and percentage of CD107A+ out of CD8+ T cells from spleen of isotype antibody- (n = 11) and α-Gal9-treated (n = 8) mice. J–L Representative contour plot and percentage of PD1+ TIM3+ cells out of CD8+ T cells (J), CD4+ TCONV (K), and TREG (L) cells from spleen of isotype antibody- (n = 11) and α-Gal9-treated (n = 8) mice. Each symbol represents an individual mouse, and statistical significance was tested by two-sided unpaired t test with Welch approximation. Boxplots represent the 25th to 75th percentiles with the median as the central line, whiskers indicate minimal and maximal value (C, D), bars plots indicate mean ± SEM (F–L). Source data are provided as a Source Data file Fig7.

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