Fig. 2: The administration of corisin induces acute kidney injury and exacerbation of kidney fibrosis in TGFβ1 transgenic mice with pre-existing renal injury.
From: Microbiota-derived corisin accelerates kidney fibrosis by promoting cellular aging
A Experimental plan for inducing acute kidney injury in TGFβ1 transgenic (TG) mice with pre-existing renal dysfunction. One group of transforming growth factor β1 (TGFβ1) TG mice (TGFβ1 TG/corisin; n = 6) received 5 mg/kg of body weight of synthetic corisin by intraperitoneal injection every two days for two weeks, and another group (TGFβ1 TG/scr. peptide; n = 6) received a similar dose of scrambled peptide following the same schedule and route of administration. B Urinary corisin, albumin, and creatinine were measured as described under materials and methods. Number of mice: TGFβ1 TG/corisin, n = 6; TGFβ1 TG/scr. peptide, n = 6. Data are presented as mean ± SEM. Statistical significance was assessed using ANOVA with the Newman-Keuls test for longitudinal data and an unpaired one-sided t-test for comparisons between two groups. *p < 0.05 vs week 0; †p = 0.02 and ‡p = 0.01 vs TGFβ1 TG/scrambled peptide group. C The urinary levels of kidney injury molecule-1 (KIM-1), liver-type fatty acid-binding protein (L-FABP), blood urea nitrogen (BUN), and creatinine were measured as described under material and methods. Number of mice: TGFβ1 TG/corisin, n = 6; TGFβ1 TG/scr. peptide, n = 6. Data are presented as mean ± SD. Statistical significance was determined using a two-sided unpaired t-test. D Plasma levels of lipopolysaccharide-induced CXC chemokine (LIX/CXCL5), macrophage inflammatory protein-2 (MIP-2), interleukin-1β (IL-1β), platelet-derived growth factor (PDGF), tissue factor (TF), and plasminogen activator inhibitor-1 (PAI-1) were measured using enzyme immunoassays. Normally distributed data are presented as mean ± SD, while skewed data are expressed as the median with interquartile range. Number of mice: TGFβ1 TG/corisin, n = 6; TGFβ1 TG/scr. peptide, n = 6. Statistical significance was assessed using a two-sided unpaired t-test for normally distributed data and the two-sided Mann-Whitney U test for skewed data. E–I Masson’s trichrome and periodic acid–Schiff staining of renal tissues from both groups of mice. Renal fibrosis was quantified using WinROOF imaging software. Scale bars indicate 100 µm in (D) and 50 µm in (F). Number of mice: TGFβ1 TG/corisin, n = 6; TGFβ1 TG/scr. peptide, n = 6. Data are presented as mean ± SD. Statistical significance was determined using a two-sided unpaired t-test. The source data are available in the Source Data file.
