Fig. 3: Treatment with the anticorisin antibody inhibits the progression of kidney fibrosis under diabetic conditions.

A Experimental plan. Diabetic TGFβ1 TG mice were divided into the following groups. One group (DM TG/anticorisin) received 10 mg/kg of body weight of anticorisin mAb by intraperitoneal injection three times a week for eight weeks, while another group (DM TG/control IgG) received a similar dose of IgG control. Following the same sampling schedule, a wild-type (WT) (n = 4) group was also included. B Blood glucose levels throughout the experiment, intraperitoneal glucose tolerance test (IPGTT), plasma creatinine, blood urea nitrogen (BUN), urine albumin/creatinine ratio, and urinary liver-type fatty acid-binding protein (L-FABP) levels. n = 5 for DM TG/control IgG and DM TG/anti-corisin; n = 4 for WT/SAL. Normally distributed data are presented as the mean, while skewed data are presented as the median. Statistical significance was assessed using ANOVA followed by either the Newman-Keuls or Dunn’s post hoc test, as appropriate; all tests were two-sided. †p < 0.05 vs WT/SAL across the same week. C, D Paraffin-embedded kidney tissue samples for collagen staining with trichrome acid. n = 5 in DM TG/control IgG and DM TG/anticorisin groups, and n = 4 in the WT/SAL group. Renal fibrosis was quantified using WinROOF. Scale bars represent 500 µm. Data are presented as mean ± SD. Statistical significance was assessed using ANOVA and the Newman-Keuls test; all tests were two-sided. E, F Paraffin-embedded kidney tissue samples for Periodic acid-Schiff (PAS) staining. Scale bars represent 50 µm. n = 5 in DM TG/control IgG and DM TG/anticorisin groups, and n = 4 in the WT/SAL group. Data are presented as mean ± SD. Statistical significance was assessed using ANOVA and the Newman-Keuls test; all tests were two-sided. G, H The plasma levels of lipopolysaccharide-induced CXC chemokine/C-X-C motif chemokine 5 (LIX/CXCL5), interleukin-1β (IL-1β), matrix metalloproteinase-2 (MMP-2), platelet-derived growth factor (PDGF), total and active transforming growth factor β1 (TGFβ1), connective tissue growth factor (CTGF), and collagen I were measured by enzyme-linked immune assays. n = 11 in DM TG/control IgG, n = 10 in DM TG/anticorisin groups, and n = 4 in the WT/SAL group. Normally distributed data are presented as mean ± SD, while skewed data are expressed as the median with interquartile range. Statistical significance was assessed using ANOVA followed by the Newman-Keuls or Dunn’s post hoc test, as appropriate; all tests were two-sided. The source data are available in the Source Data file.