Fig. 6: Mitochondrial morphology and functional capacity of the choroid plexus as a function of age.
a Representative TEM images of mitochondria at all ages. Scale bar: 200 nm. Quantification of mitochondrial (mito) density (b), cross-sectional area (c), and circularity (d), n = 3 rats (each dot represents the average of seven images per animal). e Representative trace of oxygen consumption (O2 flux, reported per mg choroid plexus tissue) in the lateral choroid plexus from a 3 M rat with compound additions marked with arrows. f Steady state O2 flux in 3 M rat following each compound addition (as illustrated in panel e), n = 5. g Maximal oxidative phosphorylation capacity (Oxphosmax) of choroid plexus as a function of age, n = 6 of each. Digi; digitonin, P; pyruvate, M; malate, G; glutamate, Succ; succinate, CytC; cytochrome C. 1 M; turquoise, 3 M; green, 6 M; orange, 12 M; red, 18 M; purple, 24 M; grey. Data are presented as mean ± SEM and statistical significance was tested with Kruskal-Wallis test followed by Dunn’s multiple comparisons test (b–d) or with one-way ANOVA followed by Tukey’s multiple comparisons test (f, g). Non-significance is not illustrated for clarity. Source data are provided as a Source Data file.
