Fig. 2: AcRR1 increases the GNS and grain yield of wheat.

a Spike shapes of the Fielder and AcRR1-OE lines. Scale bar, 2 cm. b Representative middle spikelet and corresponding disassembled spikelet from the Fielder and AcRR1-OE lines. Scale bar, 1 cm. c The number of grains along the spike. Grain number per spikelet (d), grain number per spike (e), number of sterile spikelets (f), thousand-grain weight (g), and grain yield per unit area (h) of the T4 AcRR1-OE lines and the Fielder at a high seeding rate (270 grains/m2) in the field. i Assessment of the effect of AcRR1 on T3 AcRR1-KNOE lines in the field. Grain number per spikelet (j), grain number per spike (k), thousand-grain weight (l) and grain yield per unit area (m) of the T3 AcRR1-KNOE and AcRR1-KNCOM lines at a high seeding rate (270 plants/m2) in the field. In (d‒h, j‒m) the data are presented as the means ± S.D.s. N represents the number of biologically independent plants. Boxes represent the median values and the first and third quartiles; whiskers represent the minimum and maximum values. A two-tailed Student’s t test was used to determine P values. Source data are provided as a Source Data file.