Fig. 5: AcRR1 promotes early heading by regulating the TabHLH25-5B‒TaFT-B1 pathway. | Nature Communications

Fig. 5: AcRR1 promotes early heading by regulating the TabHLH25-5B‒TaFT-B1 pathway.

From: AcRR1 of Agropyron cristatum boosts wheat yield by regulating grain number per spike and heading date

Fig. 5

a Distribution of candidate AcRR1-binding regions across the wheat genome, as determined by ChIP-seq. b Venn diagram showing the overlap of the 772 genes bound by AcRR1 with the DEGs identified by RNA-seq of the young spikes and leaves. c Expression of TabHLH25-5B in AcRR1-OE lines. d Peak distribution of AcRR1-binding sites for TabHLH25-5B. e ChIP‒qPCR assay of the binding of AcRR1 to TabHLH25-5B. f Direct binding of AcRR1 to the TabHLH25-5B 3’UTR according to the DLR assay. g EMSA of the binding of AcRR1-His to the TabHLH25-5B 3’UTR. h Comparison of the heading date of F2 plants derived from a cross between the AcRR1-OE line and the Taft mutant. Scale bar, 10 cm. i Direct binding of TabHLH25-5B to the TaFT-B1 promoter, as analyzed by the DLR assay. j EMSA of the binding of TabHLH25-5B-GST to the E-box in the TaFT-B1 promoter. The data are presented as the means ± S.D.s. In (c, e), n = 3 biologically independent replicates. In (f, i), n = 4 biologically independent replicates. A two-tailed Student’s t test was used to determine P values. Source data are provided as a Source Data file.

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