Fig. 1: Dysregulated thymopoiesis of 2D2 TCR transgenic mice.
A Contour plots show the CD4 versus CD8 profile of total thymocytes in WT, AND, and 2D2 transgenic mice. The results are representative of at least 6 independent experiments. B Bar graphs summarize the total thymocyte numbers of WT (n = 16), AND (n = 10), and 2D2 (n = 12) transgenic mice from 11 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. ***, P < 0.001; NS, not significant. One-Way Anova P < 0.0001. Data are presented as mean values ± Standard Deviation (SD). C Bar graphs show the frequencies and numbers of DN thymocytes in WT (n = 16), AND (n = 9), and 2D2 (n = 12) transgenic mice as summarized from 11 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. *, P < 0.05; **, P < 0.01; and ***, P < 0.001; NS, not significant. DN thymocytes frequency One-Way Anova P < 0.0001; DN thymocytes cell number One-Way Anova P = 0.0071. Data are presented as mean values ± SD. D Histograms show TCRβ expression on the DN thymocytes of AND (left) and 2D2 (right) transgenic mice. The bar graph summarizes the frequency of TCRβ+ DN thymocytes with 4 mice per group. Unpaired two-tailed Student’s t-test was used to calculate P values. ***, P < 0.001. Data are presented as mean values ± SD. E Histograms show expression of the clonotypic TCRα Vα3.2 (left) and TCRβ Vβ11 (right) expression (black) on DN thymocytes in 2D2 transgenic mice. The results are representative of 3 independent experiments. Ctrl Ab: control antibody. F Contour plots show the CD4 versus CD8 profiles of TCRβhi mature thymocytes from WT, AND, and 2D2 transgenic mice. Results are representative of 6 independent experiments. G Bar graphs show the frequencies of TCRβhi DP, CD4SP, and DN thymocytes from WT (n = 15), AND (n = 10), and 2D2 (n = 12) transgenic mice as summarized from 11 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. **, P < 0.01; and ***, P < 0.001; NS, not significant. TCRβhi DP One-Way Anova P < 0.0001; TCRβhi CD4SP One-Way Anova P < 0.0001; TCRβhi DN One-Way Anova P < 0.0001. Data are presented as mean values ± SD. H Histograms show CCR7 and MHC-I (H-2KbDb) expression on TCRβhi DP versus TCRβhi DN thymocytes of 2D2 transgenic mice. Results are representative of at least 3 independent experiments. I Histograms show CD103 and β7 expression on TCRβhi-gated DP, CD4SP, and DN thymocytes of AND and 2D2 transgenic mice. Results are representative of at least 7 independent experiments. J Histograms show integrin α4 and β1 expression on TCRβhi DN thymocytes of AND and 2D2 transgenic mice. Results are representative of at least 3 independent experiments.
