Fig. 2: CD103 overexpression impairs thymic development of 2D2 transgenic mice.
A Cell surface expression of integrin CD103 (top) and α4 (bottom) in different thymocyte populations of WT, CD103Tg, and CD103Tgβ7Tg mice. Histograms are representative of at least 10 independent experiments. Ctrl Ab: control antibody. B Histograms show CD103 expression on the DP, CD4SP, and DN thymocytes of WT, 2D2, and 2D2.CD103Tgβ7Tg mice. Results are representative of at least 3 independent experiments. Ctrl Ab: control antibody. C Bar graph summarizes the total thymocyte numbers in WT (n = 12), 2D2 (n = 11), and 2D2.CD103Tgβ7Tg (n = 6) mice. Overexpression of CD103 and β7 results in 0.61-fold decrease in thymocyte numbers in 2D2 mice. Results are a summary of 6 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. **, P < 0.01; and ***, P < 0.001. One-Way Anova P < 0.0001. Data are presented as mean values ± SD. D Contour plots (top) show CD4 versus CD8 profiles of WT, 2D2, and 2D2.CD103Tgβ7Tg thymocytes. Bar graph summarizes the frequencies of DP, CD4SP, and DN cells among total thymocytes of WT, 2D2, and 2D2.CD103Tgβ7Tg mice (bottom). Contour plots are representative and bar graphs show the summary of 5 independent experiments with a total of 6 mice per group. Paired two-tailed Student’s t-test was used to calculate P values. *, P < 0.05; **, P < 0.01, and ***, P < 0.001; NS, not significant. DP One-Way Anova P = 0.0003; CD4SP One-Way Anova P < 0.0001; DN One-Way Anova P = 0.0018. Data are presented as mean values ± SD. E Contour plots identify positively selected cells among total thymocytes by their CCR7 versus TCRβ expression (top). Bar graph summarizes the frequencies of CCR7hiTCRβhi thymocytes of WT (n = 7), 2D2 (n = 5), and 2D2.CD103Tgβ7Tg (n = 8) mice (bottom). Contour plots are representative, and the bar graph shows the summary of 3 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. *, P < 0.05, ***, P < 0.001. One-Way Anova P < 0.0001. Data are presented as mean values ± SD. F Contour plots show CD69 versus MHC-I expression of CCR7hiTCRβhi thymocytes from WT, 2D2, and 2D2.CD103Tgβ7Tg mice. Based on the differential expression of CD69 and MHC-I, three distinct populations are identified and their frequencies are shown. Contour plots are representative of 3 independent experiments. Bar graph summarizes the frequency of M1 population in WT, 2D2 (n = 3), and 2D2.CD103Tgβ7Tg (n = 5) mice. Unpaired two-tailed Student’s t-test was used to calculate P values. **, P < 0.01. Data are presented as mean values ± SD.
