Fig. 4: Negative selection in OT-II and AND TCR transgenic mice.

A Contour plots (top) show CD4 versus CD8 profiles of WT, AND, and AND.CD103^Tgβ7^Tg thymocytes. Bar graphs (bottom) show the frequencies of DN and total thymocyte numbers of AND (n = 4) and AND.CD103^Tgβ7^Tg (n = 6) mice as summarized from 4 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. *, P < 0.05 and ***, P < 0.001. Data are presented as mean values ± SD. B Contour plots show the CXCR4 versus CCR7 profiles of AND and AND.CD103^Tgβ7^Tg thymocytes (left), and the CD4 versus CD8 profile of CCR7^int thymocytes of AND.CD103^Tgβ7^Tg mice (right). Results are representative of 5 independent experiments. C Contour plots (top) show CD4 versus CD8 profiles of WT, OT-II, and OT-II.CD103^Tgβ7^Tg thymocytes. Bar graphs (bottom) show the frequencies of DN and total thymocyte numbers of OT-II (n = 9) and OT-II.CD103^Tgβ7^Tg (n = 6) mice as summarized from 8 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. ***, P < 0.001. Data are presented as mean values ± SD. D Contour plots show the CXCR4 versus CCR7 profiles of OT-II and OT-II.CD103^Tgβ7^Tg thymocytes (left), and the CD4 versus CD8 profile of CCR7^int thymocytes of OT-II.CD103^Tgβ7^Tg mice (right). Results are representative of 6 independent experiments. E Contour plots show the CD69 versus CCR7 profiles of WT, OT-II, and OT-II.CD103^Tgβ7^Tg thymocytes, identifying six distinct populations whereby population VI is outlined in red. Results are representative of 3 independent experiments. F Histograms show surface PD-1 (left) and nuclear Helios expression (right) of population VI cells of WT, OT-II, and OT-II.CD103^Tgβ7^Tg thymocytes. Results are representative of 3 independent experiments. G Contour plots of total thymocytes (left) show the CD69 versus CCR7 profiles of WT and HY TCR transgenic male mice (HY^male), whereby population VI is outlined in red. The CD4 versus CD8 profile (right) was further determined on population VI cells of HY^male mice. Results are representative of 2 independent experiments. H Bar graph shows the frequency of population VI cells among WT (n = 6) and HY^male (n = 4) mice. Results are a summary of 2 independent experiments. Unpaired two-tailed Student’s t-test was used to calculate P values. ***, P < 0.001. Data are presented as mean values ± SD. I Histograms show surface CD103 (left) and PD-1 expression (right) on population VI cells in HY^male thymocytes. Results are representative of 2 independent experiments.