Fig. 4: Inhibition of glycolysis and inflammation by pHO-1 delivery. | Nature Communications

Fig. 4: Inhibition of glycolysis and inflammation by pHO-1 delivery.

From: Near-infrared light-driven metabolic reprogramming of synoviocytes for the treatment of rheumatoid arthritis

Fig. 4

a The expression level of HO-1 in LPS-stimulated RAW264.7 cells after the pHO-1 transfection. Representative blots of three independent experiments with similar results are shown. b, c ECAR and OCR of LPS-stimulated RAW264.7 cells after the pHO-1 transfection. Data are presented as mean ± SD (n = 5 independent samples). d–f Relative mRNA levels of Glut1, Hk2 and Ldha in LPS-stimulated RAW264.7 cells after the pHO-1 transfection. g The macrophage phenotype after the pHO-1 transfection. Nuclei, blue (DAPI); CD206, green; INOS, red. Scale bar: 10 μm. Representative images of three biologically independent experiments with similar results are shown. h, i Relative mRNA levels of Il1b and Il10 in LPS-stimulated RAW264.7 cells after the pHO-1 transfection. LPS- represents the RAW264.7 cells without lipopolysaccharide stimulation, and LPS+ groups represent the RAW264.7 cells treated with lipopolysaccharide (0.1 μg/ml) for 24 h (d–f, h, i). Data are presented as mean ± SD (d–f, h, i, n = 3 independent experiments), and one-way ANOVA with LSD test is used for statistical analysis. Source data are provided as Source Data files.

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