Fig. 1: Structure determination of human MPC1-2 complex.
From: Structure of human mitochondrial pyruvate carrier MPC1 and MPC2 complex
a A brief schematic diagram of pyruvate transport and metabolism. Pyruvate is transported into the matrix by the Mitochondrial Pyruvate Carrier (MPC) and subsequently converted to acetyl-CoA by the pyruvate dehydrogenase (PDH) complex or oxaloacetate by the pyruvate carboxylase (PC). OMM outer mitochondrial membrane, IMM inner mitochondrial membrane, IMS mitochondrial intermembrane space, TCA tricarboxylic acid. Created in BioRender. Qi, X. (2025) https://BioRender.com/ahg95gr. b Serial dilutions of wild-type (WT) and mpc2Δmpc3Δ yeast strains spotted on medium lacking leucine (-URA-LEU). In yeast, the de novo synthesis of leucine begins with mitochondrial pyruvate, therefore growth on plates lacking leucine is indicative of a functional MPC. The cells were transformed with empty vector (EV), wild-type human MPC1-2 or MPC1-2Cryo. c Cryo-EM map of MPC1-2Cryo–UK5099. Maltose-binding protein (MBP), designed ankyrin-repeat protein off7 (DARPin), MPC1, MPC2 and UK5099 are shown as violet, turquoise, light blue, blue and orange, respectively.
