Fig. 2: Structure of MPC1-2 in the matrix-open state.
From: Structure of human mitochondrial pyruvate carrier MPC1 and MPC2 complex
a Structural alignment of MPC1 and MPC2 from MPC1-2Cryo–UK5099 structure performed using PyMOL, and the topology of MPC. MPC1 and MPC2 are shown in light blue and blue, respectively. The flexible N-terminal helices are indicated in gray. Structural diagram is created in BioRender. Qi, X. (2025) https://BioRender.com/ihdr6wh. b Overall structure of MPC1-2 form the IMM side view and top view. UK5099 is shown as orange sticks. c The electrostatic surface representation of MPC1-2 complex indicating a matrix-open state. d The nonpolar side chains between MPC1-TM1 and MPC2-TM2, MPC1-TM2 and MPC2-TM1 in the matrix-open state. e The hydrogen bonds (shown as dashed lines with distances (Å) labeled) between MPC1 and MPC2 at IMS side. f Co-immunoprecipitation of MPC1 and 2 complex. MPC1-Flag and MPC2-His were co-transfected to HEK293T cells and the MPC1-2 complexes were precipitated by anti-Flag M2 resin. MPC1 and MPC2 were detected by anti-Flag and anti-His antibodies. β-tubulin was used as an internal control. The experiment was repeated at least three times with similar results.
