Fig. 3: UK5099 binds to MPC1-2 complex on the matrix side.
From: Structure of human mitochondrial pyruvate carrier MPC1 and MPC2 complex
a Binding of UK5099 to MPC1-2 complex. The cryo-EM density of UK5099 is shown as green mesh isolated from the composite map of MPC1-2Cryo–UK5099 in PyMOL at contour level = 10. b Interaction details between MPC1-2 and UK5099. The hydrogen bonds are indicated as dashed lines with distances (Å) labeled. c The binding affinities of UK5099 to MPC1-2 complex and its variants measured by Isothermal Titration Calorimetry (ITC). The ITC binding curves for wild-type (WT), MPC2K49A and MPC2N100A are colored in green, magenta and blue, respectively. ND not detected. d Serial dilutions of wild-type (WT), mpc2Δmpc3Δ and mpc1Δ yeast strains spotted on medium lacking leucine (-URA-LEU). The mpc2Δmpc3Δ cells were co-transformed with wild-type MPC1 and wild-type MPC2, MPC2K49A or MPC2N100A. The mpc1Δ cells were transformed with wild-type MPC1, MPC1N33A, MPC1Y62F or MPC1H84A.
