Fig. 4: Structure of MPC1-2 in the IMS-open state.
From: Structure of human mitochondrial pyruvate carrier MPC1 and MPC2 complex
a Serial dilutions of wild-type (WT) and mpc2Δmpc3Δ yeast strain spotted on medium lacking leucine (-URA-LEU). The cells were co-transformed with wild-type MPC1 and wild-type MPC2 or MPC2W82F. b Cryo-EM map of MPC1-2Cryo–W82F. MBP, DARPin, MPC1 and MPC2 are shown as violet, turquoise, light green, and green, respectively. c Overall structure of MPC1-2W82F from the IMM side view. MPC1 and MPC2 are shown as light green and green, respectively. d The electrostatic surface representation of MPC1-2 complex indicating a IMS-open state. e The nonpolar side chains between MPC1-TM1 and MPC2-TM2, MPC1-TM2 and MPC2-TM1 in the IMS-open state. f Interaction details between MPC1 and MPC2 within the center of the TMs on the matrix side. The hydrogen bond between MPC1-Asn77 and MPC2-Tyr85 is indicated as a dash line with distance (Å) labeled.
