Fig. 1: Characterization of the recombinant influenza A/American Wigeon/South Carolina/22-000345-001/2021 (A/AW/SC/2021) hemagglutinin (HA) antigen. | Nature Communications

Fig. 1: Characterization of the recombinant influenza A/American Wigeon/South Carolina/22-000345-001/2021 (A/AW/SC/2021) hemagglutinin (HA) antigen.

From: Single-dose avian influenza A(H5N1) Clade 2.3.4.4b hemagglutinin–Matrix-M® nanoparticle vaccine induces neutralizing responses in nonhuman primates

Fig. 1

a Linear diagram of the full-length HA from A(H5N1) clade 2.3.4.4b A/AW/SC/2021 with the polybasic cleavage site deletion ΔKRRK. The diagram shows the HA construct from N- to C-terminus including the globular head region containing the signal peptide (SP, tan), and the stalk/stem region containing the fusion peptide (FP, red), linker (orange), transmembrane domain (TMD, yellow), and cytoplasmic tail (CT, white) structural elements. b Reduced SDS-PAGE gel with Coomassie blue staining of purified recombinant A(H5N1) A/AW/SC/2021 HA and western blot using an anti-H5N1 HA primary antibody to confirm the identity of the main protein product (experiment repeated 3 times). c Representative electron micrographs of negative-stained A(H5N1) A/AW/SC/2021 HA proteins as pre-fusion HA-trimers (yellow arrows) anchored in detergent (PS80; white arrows) micelles. Negative staining TEM-2D class average images of A/AW/SC/2021 HA nanoparticles shown with one or two HA trimers associated with a detergent micelle. d Representation of a 3D-reconstructed model of A/AW/SC/2021 HA from cryoEM map at 4.2 Å (left). A molecular model for A/AW/SC/2021 HA was generated using PDB ID 6HJR as a template and superimposed into EM density to represent an intact full-length A/AW/SC/2021 HA nanoparticle. Source data are provided in a Source Data file.

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