Fig. 2: Structural and biophysical characterization of A(H5N1) A/AW/SC/2021 HA–Matrix nanoparticle (MNP) formation. | Nature Communications

Fig. 2: Structural and biophysical characterization of A(H5N1) A/AW/SC/2021 HA–Matrix nanoparticle (MNP) formation.

From: Single-dose avian influenza A(H5N1) Clade 2.3.4.4b hemagglutinin–Matrix-M® nanoparticle vaccine induces neutralizing responses in nonhuman primates

Fig. 2

NS-TEM 3D reconstructions of (a) an A(H5N1) A/AW/SC/2021 HA nanoparticle and (b) a Matrix-M nanoparticle. c High-performance size-exclusion chromatography (HP-SEC) analysis of a mixture of 30 µg/mL A/AW/SC/2021 HA nanoparticles with 150 µg/mL Matrix-M at time 0 and after incubation at room temperature for 2–12 h (hr), showing the percentage of H5-MNP formation as % bound across time. d, e NS-TEM landscape characterizing H5N1 interaction with Matrix-M after co-mixing HA:Matrix-M (120 µg/mL and 75 µg/mL) and the formation of H5-MNPs. Representative micrographs are shown at T0 and T24 along with 2D class average (below) at respective time points (experiment repeated 3 times). Yellow arrows indicate HA-PS80 nanoparticles, purple arrows indicate H5-MNP formation. In (d), we show a micrograph of H5N1-nanoparticle (NP) distribution at time 0 (T0). In (e), we show a micrograph of H5N1-MNP formation at time 24 h (T24hr). f A 3D model of an H5-MNP, with Matrix-M cryoEM map shown in grayscale and H5 trimers (PDB 6HJR shown in blue) docked onto the Matrix-M to mimic the MNP formation from 2D classification. Source data are provided as a Source Data file.

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