Fig. 1: Enrichment of CD11c + CD8 + T cells in decidua of EPL patients.
From: Enrichment of decidual CD11c + CD8 + T cells with altered immune function in early pregnancy loss
A t-SNE visualization of dimensionality reduction clustering plot showing three main clusters of decidual CD8 + T cells from 3 EPL patients and 3 HC women. Different colors represent distinct cell clusters and disease states (see legend). B t-SNE visualization distribution plot of marker genes used to identify three major decidual CD8 + T cell types. C Proportions (left) and numbers (right) of three major subsets of decidual CD8 + T cells from EPL and HC samples. D Volcano plot showing differentially expressed genes in decidual CD8 + T cells from EPL and HC. |log2FC | > 1 and P value < 0.05 were considered significant. E Violin plot depicting higher expression of ITGAX in EPL compared to HC decidual CD8 + T cells. ****P < 0.0001. F Violin plot displaying differential expression of selected marker genes in CD11c + CD8 + T cells from EPL and HC decidua. ****P < 0.0001. G GO and KEGG pathway enrichment analysis plot of CD11c + CD8 + T cells from EPL and HC decidua. H Typical gating strategy for CD11c + CD8 + T cells in early pregnancy decidua and PBMC. I Flow cytometry analysis and quantification of CD11c + CD8 + T cells in PBMC and decidua from healthy early pregnant women (n = 10). J Flow cytometry detection of CD11c + CD8 + T cell frequency in decidua from HC (n = 18) and EPL patients (n = 20). Left panel shows typical flow cytometry gating of CD11c on decidual CD8 + T cells. K Typical immunofluorescence staining image of CD11c (green), CD8 (red), and DAPI (blue) in decidua from HC and EPL patients. White arrows indicate a single CD11c + CD8 + T cell. Scale bar = 50 µm. Representative results from three independent biological replicates. EPL early pregnancy loss, HC healthy control, GO gene ontology, KEGG kyoto encyclopedia of genes and genomes, PBMC human peripheral blood mononuclear cell. The P value was obtained by two-tailed unpaired Student’s t-test (I, J), and data are presented as mean ± SD. Source data are provided as a Source Data file.
