Fig. 4: Inhibition of trophoblast invasion by decidual CD11c + CD8 + T cells from EPL patients via NLRP3/Caspase-1/GSDMD-mediated pyroptosis.
From: Enrichment of decidual CD11c + CD8 + T cells with altered immune function in early pregnancy loss
A Schematic representation of sorting CD8 + T cells from EPL and HC decidua and their in vitro co-culture experiment with HTR-8/SVneo trophoblasts. B Typical flow cytometry gating strategy for sorting CD8 + T cells from human decidua. C Western blotting examining the impact of human decidual CD8 + T cells on protein levels of NLRP3, Caspase-1, and GSDMD in HTR-8/SVneo trophoblasts (n = 3 biologically independent experiments). D Transwell assays assessing the effect of human decidual CD8 + T cells on HTR-8/SVneo trophoblast invasion capabilities (n = 3 biologically independent experiments). Scale bar = 100 µm. E Quantification and representative images of placental organoid formation in the presence of human decidual CD8 + T cells (n = 3 biologically independent experiments). Scale bar = 100 µm. EPL early pregnancy loss, HC healthy control. The P value was obtained by one-way ANOVA (C–E), and data are presented as mean ± SD. Source data are provided as a Source Data file.
