Fig. 5: IFN-γ modulates HTR-8/SVneo trophoblast behavior by inducing pyroptosis via activation of the NLRP3/Caspase-1/GSDMD pathway.
From: Enrichment of decidual CD11c + CD8 + T cells with altered immune function in early pregnancy loss
A, B HTR-8/SVneo trophoblasts were treated with or without IFN-γ (10 ng/ml), and qPCR and western blotting were used to detect the mRNA (A) and protein (B) expression levels of NLRP3, Caspase-1, and GSDMD in HTR-8/SVneo trophoblasts (n = 3 biologically independent experiments). C PI staining shows the proportion of PI-positive (red) cells in HTR-8/SVneo trophoblasts treated with or without IFN-γ (10 ng/ml) alone, or pretreated with MCC950 (10 µM) for 1 hour before IFN-γ treatment (n = 3 biologically independent experiments). Scale bar = 100 µm. D, E Transwell and wound healing assays were performed to evaluate the effects of with or without IFN-γ (10 ng/ml) treatment alone, or pre-treatment with MCC950 (10 µM) for 1 hour before IFN-γ treatment, on the invasion (D) and migration (E) capabilities of HTR-8/SVneo trophoblasts (n = 3 biologically independent experiments). Scale bar = 100 µm (D), 200 µm (E). F After 24 hours of in vitro culture, human villous explants were treated with or without IFN-γ (10 ng/ml) or pretreated with MCC950 (10 µM) for 1 hour before IFN-γ treatment. The outward growth of EVT from the explants (outlined in red) was assessed at 48 hours (n = 3 biologically independent experiments). Scale bar = 50 µm. qPCR quantitative polymerase chain reaction, PI propidium iodide, EVT extravillous trophoblast. The P value was obtained by two-tailed unpaired Student’s t-test (A), one-way ANOVA (C–F), and data are presented as mean ± SD. Source data are provided as a Source Data file.
