Fig. 6: IFN-γ induces pyroptosis in human primary EVT cells, leading to reduced invasion and migration.
From: Enrichment of decidual CD11c + CD8 + T cells with altered immune function in early pregnancy loss
A Immunofluorescence staining for CK7 (green) and HLA-G (red) was used to verify the purity of human primary EVT cells. Scale bar = 20 µm. Representative results from three independent biological replicates. B PI staining shows the proportion of PI-positive (red) cells in human primary EVT cells treated with or without IFN-γ (10 ng/ml) alone, or pretreated with MCC950 (10 µM) for 1 hour before IFN-γ treatment (n = 3 biologically independent experiments). Scale bar = 100 µm. C Scanning electron microscopy reveals the cell morphology of human primary EVT cells with or without IFN-γ (10 ng/ml) treatment, or pretreated with MCC950 (10 µM) for 1 hour before IFN-γ treatment. Scale bar = 20 µm. Representative results from three independent biological replicates. D, E Transwell and wound healing assays were conducted to assess the effects of with or without IFN-γ treatment alone, or pretreated with MCC950 (10 µM) for 1 hour before IFN-γ treatment, on the invasion (D) and migration (E) capabilities of human primary EVT cells (n = 3 biologically independent experiments). Scale bar = 100 µm (D), 200 µm (E). EVT extravillous trophoblast, CK7 cytokeratin 7, HLA-G human leukocyte antigen-G, PI propidium iodide. The P value was obtained by one-way ANOVA (B, D, E), and data are presented as mean ± SD. Source data are provided as a Source Data file.
