Fig. 7: Alterations in the frequency and function of CD11c + CD8 + T cells in mice decidua affect early pregnancy maintenance.
From: Enrichment of decidual CD11c + CD8 + T cells with altered immune function in early pregnancy loss
A Representative images of a pregnant uterus from mice in various groups: NP (normal pregnancy group), 4-1BB (normal pregnant mice injected intravenously with 5 mg/kg anti-mouse 4-1BB antibody at E7.5), and AP (abortion-prone group). B–D Quantitative statistics of embryo resorption rate (B), number of viable embryos per uterus (C), and average body weight of pregnant mice at E12.5 (D) for each group (n = 10). E Flow cytometry analysis of CD11c expression in CD8 + T cells isolated from mice decidua in each group (n = 10). F Expression of CD69, CD103, and PD-1 in CD11c + CD8 + T cells from mice decidua in each group (n = 10). G Expression of cytotoxicity-related molecules (CD107a, granzyme B, perforin) and cytokine (IFN-γ) in CD11c + CD8 + T cells from mice decidua in each group (n = 10). H HE and immunofluorescence staining to observe the infiltration depth of CK7+ trophoblast layer (green) into the decidua of mice uterus. The relative depth of EVT infiltration into the uterus is calculated as the ratio of the depth of CK7+ trophoblast layer (L1, red) to the total depth (L2, orange). I Quantitative statistics of the relative depth of EVT infiltration into the uterus (n = 3 biologically independent experiments). Scale bar = 500 µm. NP normal pregnancy, AP abortion prone, CK7 cytokeratin 7, EVT extravillous trophoblast. The P value was obtained by two-tailed unpaired Student’s t-test (B–G, I), and data are presented as mean ± SD. Source data are provided as a Source Data file.
