Fig. 7: Septal AVP release is reduced in Shank3B+/- mice during social exploration and aggression. | Nature Communications

Fig. 7: Septal AVP release is reduced in Shank3B+/- mice during social exploration and aggression.

From: Impaired vasopressin neuromodulation of the lateral septum leads to social behavior deficits in Shank3B+/- male mice

Fig. 7

a WT and Shank3B+/- mice bilaterally injected with AAV9hSyn.AVP0.3 and implanted with an optical ferrule in the lateral septum (LS) (Created in BioRender. Leroy, F. (2025) https://BioRender.com/j3posib). b Image of trace of the optical ferrule tip in LS (scale bar: 400 μm). c Experimental timeline. d Schematic of the resident-intruder test (Created in BioRender. Leroy, F. (2025) https://BioRender.com/vyq5lxl). e Peri-stimulus time histogram (PSTH) during social exploration for WT and Shank3B+/- mice (7 mice/group). f Areas under the curve of the PSTH before and during social exploration for WT mice (7 mice. Paired t test: p = 0.009). g Peak amplitudes of the PSTH before and during social exploration for WT mice (7 mice. Paired ttest: p = 0.006). h Areas under curve for Shank3B+/- mice (7 mice. Paired t test: p = 0.07). i Peak amplitudes for Shank3B+/- mice (7 mice. Paired t test: p = 0.9). j Areas under the curve during social exploration WT vs. Shank3B+/- mice (7 mice/group. Unpaired t test: p = 0.001). k Peak amplitudes during social exploration for WT vs. Shank3B+/- mice (7 mice/group. Unpaired t test: p = 0.4). l Linear SVM decoder performance (7/5 mice. Wilcoxon signed-rank test WT: p = 0.01; Shank3B+/-: p = 0.91; WT vs. Shank3B+/-: p = 0.00). m PSTH during biting for WT vs. Shank3B+/- mice (6/4 mice/group). n Areas under cthe urve before and during biting for WT mice (6 mice. Paired t test: p = 0.03). o Peak amplitudes during pre-biting vs biting for WT mice (6 mice. Paired t test: p = 0.004). p Areas under curve for Shank3B+/- mice (4 mice. Paired t test: p = 0.14). q Peak amplitudes for Shank3B+/- mice (4 mice. Paired t test: p = 0.26). r Areas under cthe urve during biting for WT vs. Shank3B+/- mice (6/4 mice. Unpaired t test: p = 0.002). s Peak amplitudes during biting (6/4 mice. Unpaired t test: p = 0.04). For the entire figure, box plots show the median (central line) and the full range (minimum to maximum) across animals. Each point is one mouse. PSTH plotted as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.

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