Fig. 8: Activation of AVPR1a or AVPR1b in LS of Shank3B+/- mice rescues sociability or social aggression respectively.
a Shank3B+/- mice implanted in the dorsal middle of the septum with a guide cannula guide for drug delivery (Created in BioRender. Leroy, F. (2025) https://BioRender.com/ouq4aao). b Image showing the mini-Ruby localization in LS (scale bar: 600 μm). c Experimental timeline. After cannula guide implantation, Shank3B+/- mice were infused with either saline, DDAVP, or AVP + SSR149415 before undergoing the sociability test. All animals were tested 3 times for sociability, with the infusion given in randomized order. Following social isolation, animals underwent 3 resident-intruder tests following the same infusions. d Schematic of the sociability test (Created in BioRender. Leroy, F. (2025) https://BioRender.com/irfm1dl). e Interaction times with empty and social cups (10 mice. Paired t test, saline condition: p = 0.8; Paired t test, DDAVP condition: p = 1; Paired t test, AVP + SSR149415 condition: p = 0.04). f Discrimination indexes for social preference (10 mice). Repeated measures one-way ANOVA followed with Tukey’s multiple comparisons test: F = 4.35, p = 0.04. Tukey’s multiple comparisons test: Saline vs. DDAVP p = 1; Saline vs. AVP + SSR149415 p = 0.02; DDAVP vs. AVP + SSR149415 p = 0.09). g Schematic of the resident-intruder test (Created in BioRender. Leroy, F. (2025) https://BioRender.com/mjn7ex8). h Proportions of mice exhibiting only social exploration, social dominance without aggression or aggression (9 mice. Chi-squared tests: Saline vs. DDAVP χ²(2) = 7.07, p = 0.03; Saline vs. AVP + SSR149415 χ²(2) = 0.53, p = 0.8; DDAVP vs. AVP + SSR149415 χ²(2) = 4.5, p = 0.1). i Number of attacks (9 mice. Repeated measures one-way ANOVA: F (2, 8) = 3.07, p = 0.1). For the entire figure, bar graphs represent mean ± s.e.m. Each point is one mouse. *p < 0.05, **p < 0.01 and ***p < 0.001. Source data are provided as a Source Data file.
