Table 1 Reagents and resources information
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
Antibodies | ||
Anti-c-fos antibody produced in rabbit | Abcam | #ab190289 RRID:AB_2737414 |
Anti-GFP antibody produced in chicken | AVES Labs | #GFP-1020 RRID:AB_10000240 |
Anti-vasopressin antibody produced in a rabbit | Merck | #PC234L RRID:AB_565256 |
Anti-RFP antibody produced in a rabbit | Rockland Antibody | #600-401-379 RRID:AB_2209751 |
Anti-VGLUT1 antibody produced in a guinea pig | Merck | #ab5905 RRID:AB_2301751 |
Goat anti-rabbit IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate | Thermo-Fisher Scientific | #A11039 RRID:AB_142924 |
Goat anti-rabbit IgG (H + L) secondary antibody, Alexa Fluor 568 conjugate | Thermo-Fisher Scientific | #A11011 RRID:AB_143157 |
Goat anti-guinea-pig IgG (H + L) secondary antibody, Alexa Fluor 594 conjugate | Thermo-Fisher Scientific | #A11076 RRID:AB 2534120 |
Goat Anti-chicken IgG (H + L) secondary antibody, Alexa Fluor 488 Conjugate | Thermo-Fisher Scientific | #A11039 RRID:AB_142924 |
In situ hybridization probes | ||
Avp in C1 | ACD Bio | #401391 |
Avpr1a in C3 | ACD Bio | #418061-C3 |
Chemicals, Peptides, and Recombinant Proteins | ||
CNO | Cayman Chemical | #16882 |
SR49059 | Tocris | #2310 |
Desmopressin | Tocris | #3396 |
[Arg8]-Vasopressin | Tocris | #2935 |
SSR149415 | Tocris | #6195 |
Experimental Models: Organisms/Strains | ||
C57BL/6 J Mus musculus | Jackson Laboratories | RRID:IMSR_JAX:000664 |
Avp-Cre+/- | Jackson Laboratories | RRID:IMSR_JAX:023530 |
Shank3B+/- | Jackson Laboratories | RRID:IMSR_JAX:017688 |
Balb/cByJ | Jackson Laboratories | RRID:IMSR_JAX:001026 |
Recombinant DNA | ||
HSV hEF1a.LSIL.GFP (HT) | Massachusetts General Hospital | #RN406 |
AAV2/1 syn.FLEX.GCaMP6f.WPRE.SV40 | Addgene | #100833-AAV1 |
AAV2/9EF1a.DIO.hChR2 (E123T/T159C).eYFP8 | Addgene | #35509-AAV9 |
AAV2/8 hSyn.DIO.hM4D(Gi)-mCherry | Addgene | #44362-AAV8 |
AAV2/1 hSyn1-DIO-eOPN3-mScarlet-WPRE | Addgene | #125713-AAV1 |
AAV2/9 GFP.U6.anti-Avp-shRNA | Vector Biolabs | #253437-shAAV50 |
AAV2/9 GFP.U6.scramble-shRNA | Vector Biolabs | 50 |
AAV9-hSyn-AVP0.3 | Yulong Li | |
Software | ||
PRISM 8 | Graphpad | 8.4.1 (455) |
Microsoft Office Word | Microsoft | 2019 16.56 |
Microsoft Office Excel | Microsoft | 2019 |
Adobe Illustrator | Adobe | 2023 |
Fiji | GPL v2 | 2.3.0/1.53 f |
Python | 3.10.2 | |
MATLAB | Mathworks | 2024b |
Guppy | Lerner Lab88 | 1.1.4 |
SimpylCellCounter | 2.0 | |
Leica Application Suite X | Leica | v3.7.4 |
ANY-maze | Stoelting Co. | 4.99 |
Bonsai | Open Ephys | 2.8.2 |
Doric Neuroscience Studio | Doric | 5.4.1.23 |
