Fig. 3: sRNA differs between high and low sperm concentration samples.

a MitosRNA with highest significance between high and low sperm concentration (>/≤16 million sperm/mL). MT-TH-His P val=0.000024, MT-TQ-Glu P val=0.000054, MT-TS1-Ser1 P val=0.0000009. P values were produced by Wilcoxon nonparametric test (two-sided). b Linear regression of MT-TS1-Ser1 comparing sum cpm and sperm concentration (R² = 0.208, P value = <0.0001, equation= Y = 0,01862*X + 15,83). c ROC of sum cpm of MT-TS1-Ser1 to differentiate between high and low sperm concentration. d Ribonucleoprotein associated sRNA with highest significance between high and low sperm concentration. RNY1 P val=0.000045, RNY3 p-val=0.00009, RNY4 p-val=0.000015. P values were produced by Wilcoxon nonparametric test (two-sided). e Linear regression comparing RNY4 and sperm concentration (R² = 0.238, P value = <0.0001, equation= Y = −0,0004823*X + 45,23). f ROC of sum cpm of RNY4 to differentiate between high and low sperm concentration. Orange=group of high sperm concentration, blue=group of low sperm concentration. Data is presented as sum of normalised sequences (cpm) for each sperm sample by indicated genomic origin. P values for Wilcoxon nonparametric t-test, *≤0.05, **≤0.001, ***≤0.0001. High sperm concentration n = 51, low sperm concentration n = 18. Line in box plot represents the median, hinges show the first and third quartiles and whiskers extend to the largest value unless values are above 1.5 times the inter-quartile range. In linear regression, coloured area represents 95% confidence interval.