Fig. 4: Sperm sRNA differs between samples of high and low fertilisation rate.
From: Small RNA in sperm–Paternal contributions to human embryo development
a Mean cpm of high and low fertilisation rate (≥/< 70%) of differentially expressed sequences from Fig. 2b (upper panel). Genomic region of chromosome 1, 146,035,680−146,035,770 from reference GRCh38. Annotation was extracted from IGV with RNAcentral genomic coordinates data version 24.0 (lower panel). b Sum cpm of all differentially expressed sequences in high and low fertilisation rate. P values as shown in figure were produced by Wilcoxon nonparametric test (two-sided). c Linear regression comparing sum cpm of differentially expressed sequences and fertilisation rate (R² = 0.01918, P value = 0.2529, equation= Y = −0,0003943*X + 65,25). d ROC of sum cpm of all differentially expressed sequences to differentiate between low and high fertilisation rate. Orange=group of high fertilisation rate, blue=group of low fertilisation rate. Data is presented as sum of normalised sequences (cpm) for each sperm sample by indicated genomic origin. High fertilisation rate n = 29, low fertilisation rate n = 40. Line in box plot represents the median, hinges show the first and third quartiles and whiskers extend to the largest value unless values are above 1.5 times the inter-quartile range. In linear regression, coloured area represents 95% confidence interval.
