Fig. 5: Sperm sRNA differs between samples of high and low rates of high-quality embryos.

a MiRNA with highest significance between high and low rate of high-quality embryos (≥/< 20%). Hsa-let-7g P val=0.000054, mir-320b/a P val=0.0067, hsa-mir-30d p-val=0.008. P values as shown in figure were produced by Wilcoxon nonparametric test (two-sided). b Linear regression comparing hsa-let-7g in sum cpm and rate of high-quality embryos (R² = 0.001, P value = 0.7903, equation= Y = -0,1167*X + 33,97). c ROC of sum cpm of hsa-let-7g to differentiate between high and low rate of high-quality embryos. d RsRNAs with highest significance between high and low rate of high-quality embryos. 28S rRNA=0.00018, 5S rRNA=0.0041, 5.8S rRNA=0.0096. P values as shown in figure were produced by Wilcoxon nonparametric test (two-sided). e Linear regression comparing 28 s rRNA in sum cpm and rate of high-quality embryos (R² = 0.0837, P value = 0.0194, equation=Y = −0,009374*X + 39,65). f ROC of sum cpm of 28 s rRNA to differentiate between high and low rate of high-quality embryos. Orange=group of high rate of high-quality embryos, blue=group of low rate of high-quality embryos. P values for Wilcoxon nonparametric t-test, *≤0.05, **≤0.001, ***≤0.0001. Data is presented as sum of normalised sequences (cpm) for each sperm sample by indicated genomic origin. High rate of high-quality embryos n = 47, low rate of high-quality embryos n = 17. Line in box plot represents the median, hinges show the first and third quartiles and whiskers extend to the largest value unless values are above 1.5 times the inter-quartile range. In linear regression, coloured area represents 95% confidence interval.