Fig. 6: mtDNA is localized in mitochondria in the Por1 (QA) strains lacking the MDL proteins.

a, b Cells lacking Por1 (a) and those lacking Por1 and one of the MDL proteins (b) but expressing Por1(WT) (a, left) or Por1(QA) (a, right; b) from their own promoters via plasmid transformation, were incubated with SYBR Green for mtDNA labeling in SCD medium at 30 °C for 4 h. Z-max projection images are shown. The boxed regions are magnified. Experiments were performed independently three times. Scale bars: 5 μm (main images), 1 μm (inset). Fluorescence intensity (F.I. (arbitrary unit)) profile of mtDNA (green) and Su9-RFP (magenta) along the orange lines in the left panels are shown in the right panels. Orange arrowheads indicate mtDNA-positive and Su9-negative signals. c A proposed model for the mtDNA loss caused by Por1(QA). The roles of Por1(QA) and Yme2 in mtDNA escape from mitochondria are not clear. Source data are provided as a Source Data file.