Fig. 1: Identification and characterization of broadly cross-reactive NA-targeting antibodies.
From: Broad neuraminidase antibodies confer protection against seasonal and avian influenza viruses
A Schematic representation of the antibodies screening process. B Binding heatmap of mAbs to recombination NAs representative of IAV and IBV strains by ELISA. Colored boxes represent the value of the optical density at 405 nm (OD405). C Phylogenetic tree of IAV and IBV NAs constructed using maximum likelihood analysis of amino acid sequences. Pentagrams indicate antibody-binding subtypes (OD405 over twice the negative control value; red: CAV-F6; yellow: CAV-F34). D Immunoglobulin variable heavy-chain and light-chain gene usage, HCDR3 and LCDR3 sequences of NA mAbs. E Half maximal inhibitory concentration (IC50) values of CAV-F34 and CAV-F6 against NAs via ELLA (orange) and MUNANA (red) assay. F–I Inhibitory activity of CAV-F34 and CAV-F6 against drug-resistant NA mutants in MUNANA assays. Data represents one of three independent experiments, shown as mean ± SD of three technical replicates.
