Fig. 6: Nano-chemical analysis of the emergence of supramolecular chirality in heterogeneous rac-polymer assemblies.

AFM Morphology maps of characteristic (a) large and (b) small racemic assembly. c AFM-IR spectra of several racemic assemblies normalized to 1273 cm⁻¹ (Supplementary Fig. 15); shading represents SE. d Score plot of PCA analysis of the normalized spectra in c with 95% confidence ellipses (CE). e Loading plot of the spectra highlighting the molecular bonds related to the variance difference. f Zoomed spectra from 1780–1600 cm⁻¹, normalized to 1646 cm⁻¹ (C=O peak); shading represents SE. g PCA Score Plot of second derivatives of spectra in f with 95% confidence ellipses (CE). h Loading plot of PC1 of the second derivatives highlighting the structural features of the small vs. large assemblies. i Schematic of the key molecular bonds used to identify and discriminate the emergency of different forms of chirality; from central chirality in molecules (CH₃), to backbone chirality in polymers (C=O at 1646 cm⁻¹), supramolecular order in assemblies (C=O at 1725 cm^-1).