Fig. 2: Chromatin accessibility landscapes recapitulate lineage trajectories in the testis.
From: Dissecting the enhancer gene regulatory network in early Drosophila spermatogenesis
a UMAP embedding of single-nucleus ATAC-seq data, colored by transcriptome-based latent time clusters, shows distinct accessibility trajectories for germline and soma. (arrows). b Heatmap of label transfer scores between RNA and ATAC datasets. c Left: Representative cluster-specific accessible regions; underlined regions are cluster-enriched. Right: total and cluster-specific accessible regions per cluster. d Left: Distribution of accessible regions by genomic annotation; solid and dashed lines represent germline and somatic regions, respectively. Right: distribution of gene-associated peaks per gene. e, f Accessibility at the zfh1 and stg loci ±20 kb, with cluster-specific tracks and temporal gene expression (insets). g, h Motif enrichment scores (normalized enrichment score, NES) across latent time for population-specific TFs in germline (g) and soma (h), identified via SCENIC+; logos indicate enriched motifs.
